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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Lab Invest. 2007 Jun 18;87(8):807–817. doi: 10.1038/labinvest.3700595

Figure 6.

Figure 6

CXCL12 stimulates accumulation of F-actin and RhoGTP at the leading edge of migrating IEC-6 epithelial sheets. IEC-6 monolayers wounded with a razor blade were incubated 20 min with or without 20 ng/ml CXCL12, or 1 μg/ml LPA as a control, fixed, permeablized, and stained for RhoGTP using Rhotekin-GST and an anti-GST antibody, or F-actin with Alexa-fluor-595-phalloidin. Photomicrographs indicated the CXCL12 stimulated accumulation of F-actin and RhoGTP at the leading edge of new wounds greater than that of unstimulated cells (no stim). Cells were examined using fluorescence microscopy at × 400. Data are representative of three independent experiments.