Figure 12.
Effects of cAMP Elevation on Surface TrkB Immunoreactivity
Approximately 5,000 purified SMNs were plated onto PDL-coated glass coverslips for 1 hr in serum-free medium containing the indicated substances prior to immunostaining the unpermeabilized cells with the anti-TrkB antibodies. The percentage of cells that were brightly surface immunoreactive for TrkB was counted in each condition. Results are means ± SEM (n = 3) of a representative experiment; this experiment was repeated twice.