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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Neuron. 1998 Oct;21(4):681–693. doi: 10.1016/s0896-6273(00)80586-3

Figure 4.

Figure 4

Effects of Neurotrophins and cAMP Elevation on MAP Kinase Translocation into the Nucleus

Acutely isolated, purified P8 RGCs or P2 SCG neurons were plated onto PDL-coated coverslips for 1 hr and then treated with the indicated substances. Plateau concentrations of neurotrophins were used (NGF was used at 20 ng/ml; BDNF was used at 50 ng/ml). cAMP was elevated by a combination of forskolin (5 μM); in addition, IBMX (0.1 mM) was added to the SCG neurons. RGCs (A) or SCGs (B) were cultured for various times in the indicated substances and then labeled to determine the percentage of cells with MAP kinase staining in their nucleus. All values are means ± SEM. (n = 3).