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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Nat Methods. 2008 Aug 31;5(10):869–872. doi: 10.1038/nmeth.1250

Figure 1. Screening for dopaminergic cell fate mutants.

Figure 1

(a, b) Transgenic strains used for the worm sorter screen. Scale bar represents 100 μm. (c) Experimental procedure of the manual screen. (d) Experimental procedure of the COPAS worm sorter screen. (e)-(j) Relative fluorescence intensity plots between red and green channels of sorted worms. Scale is defined by the sorting parameters (see Supplementary Methods). Each dot corresponds to a single worm. (e) Profile of a non-mutagenized population of the sorting strain. (f) Example profile of a mutagenized population of worms. Red arrows indicate individual animals that fall into the manually-set sorting window. (g-j) Profiles of homogenous populations of retrieved mutants: (g) ot344 (0 out of 8 cells expressing gfp), (h) ot337 (2-4 out of 8 cells expressing gfp), (i) ot406 (4-6 out of 8 cells expressing gfp), and (j) ot346 (7-9 out of 8 cells expressing gfp). The triangle area at the bottom right of each panel is an example of a sorting region chosen to demonstrate the profile differences between various populations. The actual sorting region used in the screening process was usually larger, to minimize the possibility of loosing mutants.