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. 2009 May 11;9:53. doi: 10.1186/1471-2229-9-53

Table 3.

Marker validation and features of new 61 UGMS markers of tea

Heterozygosity **
Locus
name * 		Primer sequence Repeat Motif Annealing temperature
(Ta)		 No. of alleles HO HE PIC Approximate size range (bp) No. of genotypes amplified
TUGMS1 F5’CTTCAAGTTGAGTTTGTCCG'
R5'CAAGGGATGGTTTTCACTTG		 (TA)13 55°C 4 0.118 0.558 0.770 85 bp–100 bp 15
TUGMS3 F5'GCGTATGGAAAAGCTGAGAA3'
R5'GAAGCAAACCACTGAGGTGA3'		 (TC)10 57°C 8 0.559 0.857 0.595 160 bp–220 bp 34
TUGMS4 F5'CCACCGACTCGATGACATAA3'
R5'GCATTGAGATTGATGGACCA3'		 (TC)11(CA)11 57°C 6 0.765 0.808 0.306 250 bp–300 bp 32
TUGMS7 F5'GGACCACTTGATTTTCAGCT3'
R5'ACGTACAATCACCACCGACT3'		 (GA)19 55°C 6 0.853 0.766 0.154 300 bp–400 bp 34
TUGMS11 F5'GGGGAGTGTTTGTTTGAATA3'
R5'TGTAGGGTTCTTTGAGGCAG3'		 (GA)22 55°C 8 0.500 0.857 0.630 190 bp–240 bp 29
TUGMS12 F5'GAAGTTTGTTGAGAGTGCTGC3'
R5'ACAGATCTAAATTTGGGGGG3'		 (TA)22 55°C 7 0.382 0.663 0.294 160 bp–200 bp 30
TUGMS13 F5'GATCTGTGTCTCTCTGTTCCC3'
R5'CCACACATCATCTTTTCCTC3'		 (TG)32(TC)24 55°C 7 0.324 0.804 0.675 185 bp–205 bp 25
TUGMS15 F5'GTTGCTTCCTTGGTGCCT3'
R5'GCGGGGACCACATYCAGTA3'		 (GA)14 55°C 15 0.500 0.871 0.692 145 bp–190 bp 30
TUGMS17 F5'GGGGAATTTCAGACAGACAC3'
R5'GCCGTTCAGTGTAGTAGATCG3'		 (TC)25 55°C 5 0.588 0.796 0.414 160 bp–200 bp 25
TUGMS18 F5'GGGGAAGAAAAAAAAAGTTG3'
R5'TTTCTGGATGTTGTAGTCGG3'		 (GA)10 55°C 4 0.059 0.583 0.921 190 bp–260 bp 13
TUGMS20 F5'GGGGAATTTCATCACTCAAAC3'
R5'AGATCGGAGTCACCGTTGTA3'		 (TC)24 55°C 4 0.235 0.748 0.727 290 bp–320 bp 21
TUGMS22 F5'GGCAGCTTCAGTTCATCTCT3'
R5'CATAAGGAAAGCTGCAAGAG3'		 (GA)13 55°C 8 0.559 0.835 0.626 140 bp–160 bp 24
TUGMS23 F5'GGGGAGCTTACAAAGAGTCA3'
R5'GTGCCGAAGAGAGGATAGAG3'		 (TC)13 55°C 8 0.618 0.839 0.503 135 bp–200 bp 31
TUGMS24 F5'CTCACTACAGCRGCAACCGC3'
R5'CCTGAATCTAGTGGGGCTTC3'		 (TC)11 55°C 5 0.235 0.727 0.640 280 bp–300 bp 24
TUGMS27*** F5'GGGGATAGTACAAACACACAAC'
R5'GCTCCTCTTTCTTCACCACTT'		 (GA)20 55°C 9 - - - 80 bp–110 bp 32
TUGMS28 F5' GTCCCCATTGCTCTTAGTTT 3'
R5' GACAATCATTGCCACCACAT 3'		 (TG)12
(GA)13 		55°C 4 0.529 0.745 0.384 170 bp–200 bp 29
TUGMS29 F5' CAAAACAGAGCCTTCATAAG 3'
R5' ATCGAGACAGAAGACAGACG 3'		 (TC)20 53°C 4 0.029 0.481 0.968 105 bp–120 bp 10
TUGMS31B F5' CTATGTACGACTCTCTGCCTG3'
R5' GTTTGTCTGGAGTTAAACGAG3'		 (TC)9 55°C 5 0.294 0.558 0.853 140 bp–170 bp 12
TUGMS33 F5'CCCTCTTCTCTCACCAGATC3'
R5'TCCCTTCTTTGCCTTCTACA3'		 (GA)10 55°C 3 0.441 0.615 0.180 150 bp–160 bp 34
TUGMS34 F5'GCCAAAATTCCATCTAGGG3'
R5'TGCAACTCGTATGTGGACC3'		 (TTC)18(GA)10 55°C 10 0.618 0.828 0.364 160 bp–200 bp 32
TUGMS35 F5'GGGGCTCTCTCTCTCTAAAG3'
R5'TGCTGTGAGAAGTAAAGGGC3'		 (TC)11 55°C 6 0.853 0.848 0.267 110 bp–140 bp 30
TUGMS36 F5'GCCAGCAAGTAAGAGAAGCT3'
R5'GTGGGTTGAGTACCAACAGG3'		 (GA)13 55°C 2 0.029 0.510 0.856 125 bp–115 bp 13
TUGMS41 F5'CCTTTCACAACAGATCCACA3'
R5'GAGCTTCCTGACGATGGTTA3'		 (GA)11 55°C 3 0.235 0.625 0.717 115 bp–120 bp 16
TUGMS42 F5'GTAGCTCGCAACACAACACC3'
R5'CTCCAACGACACACTCTCTG3'		 (GA)23
(GA)11 		55°C 7 0.588 0.860 0.581 100 bp–180 bp 29
TUGMS43A F5'CATTTCCTTCTCACCCCTAC3'
R5'GTGGGTGTGGGACTTGAATA3'		 (GA)11 55°C 4 0.264 0.576 0.842 150 bp–170 bp 13
TUGMS44 F5'GTGTTGGGAGTGTTGCTGAA3'
R5'ACCACCTGATTCGACATCTC3'		 (GA)12 55°C 4 0.118 0.478 0.353 300 bp–360 bp 25
TUGMS45 F5'GGGGATTGTTGAAGTTTCTC3'
R5'CTTCACCCATATCTTCCAAA3'		 (GA)20 55°C 2 0.118 0.555 0.764 148 bp–150 bp 16
TUGMS46*** F5'GGGTTCAGTCGCAGCAAA3'
R5'GAGGAGTTCTTCTTGCGTCT3'		 (TC)13 55°C 10 - - - 98 bp–120 bp 34
TUGMS48 F5'TCGGGCAACCACCATATATA3'
R5'CTTTTCCCACCAGACAAGAA3'		 (GA)14 55°C 7 0.441 0.880 0.755 100 bp–135 bp 28
TUGMS50 F5'GGGGATTCATCTCTGAACAC3'
R5'GGAGAGAGTGAGAGCTTTGG3'		 (TC)11 55°C 2 0.059 0.527 0.932 168 bp–175 bp 12
TUGMS51 F5'CCAGACTCATCGCAGAAATC3'
R5'GGTTGGGTGAGGAGGAATAG3'		 (GA)11 55°C 7 0.765 0.792 0.353 145 bp–170 bp 32
TUGMS52 F5'GAACCAACCCAGTCTATACTCC3'
R5'AGCACACGCCATCCAATC3'		 (GA)14 55°C 16 0.794 0.909 0.622 90 bp–120 bp 34
TUGMS58A F5'TTCTTCCTCTTCTTTGGTGG3'
R5'AGAGGGTGAAGAGGAAGTTG3'		 (TCC)14 55°C 4 0.647 0.678 0.210 90 bp–110 bp 31
TUGMS58B F5'CAACTTCCTCTTCACCCTCT3'
R5'GCTGAAGAGAACGGTGAAGA3'		 (TCG)28 55°C 2 0.059 0.216 0.124 140 bp–160 bp 31
TUGMS59 F5'CACCTTCATCTTCACCTTCC3'
R5'TGAGTCTGCTCGTAGGTGAG3'		 (TGG)9 55°C 3 0.382 0.454 0.018 168 bp–180 bp 25
TUGMS63 F5'CAAGGTAAAGGACATGCACC3'
R5'GTCCTCAGAAGCCATCGAA3'		 (CCG)6 55°C 2 0.177 0.613 0.568 150 bp–155 bp 22
TUGMS64 F5'TGCAGGGGAGATGAATTAAC3'
R5'ACCTGCATTTCCCAGTCTT3'		 (CAG)9 55°C 5 0.382 0.775 0.605 280 bp–320 bp 24
TUGMS66 F5'AATGGTTGGGTAAGCCTCT3'
R5'TGACCAACAACGGATCACA3'		 (TTA)8 55°C 4 0.441 0.644 0.231 220 bp–320 bp 29
TUGMS70 F5'ATCAGACGATGTACCGAAGAG3'
R5'CGAACGTGAATGTAATCAGG3'		 (CAA)15 55°C 2 0.029 0.504 0.782 180 bp–190 bp 14
TUGMS71 F5'AGCAGCAAGTGTCGTTTACA3'
R5'GCAGAAATGAGAGAAGGAGG3'		 (GAA)8 55°C 3 0.235 0.511 0.204 240 bp–320 bp 30
TUGMS72 F5'CCAGCTCGATAGCATCTACA3'
R5'CACTATCCAAATCCATCGC3'		 (ATG)10 55°C 2 0.559 0.396 0.072 198 bp–205 bp 28
TUGMS73 F5'GTCAAGACGCCCACTACAGT3'
R5'GACTGTGTAACCTGCCAAGAC3'		 (TAA)12 55°C 9 0.677 0.907 0.694 150 bp–220 bp 32
TUGMS74 F5'CACCCCCTTCCTATTCAAA3'
R5'AGGTGGTCACTTCTTCAACG3'		 (CCA)9 55°C 6 0.706 0.900 0.676 170 bp–200 bp 32
TUGMS75 F5'GGTGATCCGATGGTGAATT3'
R5'ACAGGAGCATCAACAGCAGG3'		 (ATG)9 55°C 4 0.265 0.293 0.032 240 bp–280 bp 34
TUGMS76 F5'AGATGAGCACAAGGAAGGAG3'
R5'CGAAGTAGTGTAGGGGAAGAA3'		 (GAA)10 55°C 2 0.441 0.618 0.652 198 bp–210 bp 16
TUGMS77 F5'CTACCCTTCTTCTCAGTTCCA3'
R5'CAGATGAAATGAAGGGCATC3'		 (CAA)10 55°C 2 0.206 0.490 0.848 132 bp–140 bp 11
TUGMS78 F5'CACCGCTTGACTAAAATGG3'
R5'AAACTATCAACCGTATGGGC3'		 (TTC)13 55°C 8 0.647 0.878 0.597 130 bp–170 bp 31
TUGMS79 F5'GGGTAATTTAAGGGTGTCCT3'
R5'AAGAGGGTGATAAGGATTCC3'		 (CCA)11 55°C 7 0.324 0.682 0.503 160 bp–260 bp 24
TUGMS82 F5'AAGTTAGAGAGAGAGAAGTGGC3'
R5'AATGCCACACCAGTCCTAG3'		 (CAT)8 55°C 6 0.412 0.691 0.344 140 bp–180 bp 30
TUGMS83 F5'GAGGATTTGGGTTTGTGAAC3'
R5'TCATTCTCTCTGGCATCACC3'		 (TGG)9 55°C 4 0.765 0.671 0.242 250 bp–600 bp 30
TUGMS84 F5'GCTAGGCATTCGAGGAGTT3'
R5'GGACTCCTCACTGCTTGAAG3'		 (ATG)34 55°C 2 0.500 0.499 0.060 220 bp–500 bp 31
TUGMS85 F5'GACGGAAAATCGAAGGC3'
R5'TCTTACTGCTCTTGGCTTCC3'		 (GAA)11 55°C 3 0.059 0.140 0.063 120 bp–140 bp 34
TUGMS87 F5'TCACATTTTCAGAGGAAGAGG3'
R5'TTAGGGTTTAGTTGTGGCTG3'		 (GAA)6 55°C 5 0.235 0.733 0.673 90 bp–125 bp 28
TUGMS90 F5'GAGGGGAAGTGTGAAAAATC3'
R5'TTGGGATTCCTTTTCTATGC3'		 (TTTG)6 55°C 4 0.412 0.696 0.607 95 bp–120 bp 18
TUGMS92 F5'TCTATCAGTTGGCTTGGTTG3'
R5'CAATCTTTCACTGGCATGAG3'		 (TTTC)13 55°C 4 0.118 0.269 0.972 145 bp–180 bp 5
TUGMS95 F55'GGCTCCTTCCTCTTCTGATC3'
R5'TGAAGTTGGGATTGAGCATG3'		 (CAAA)6 55°C 5 0.412 0.777 0.596 120 bp–150 bp 23
TUGMS98 F5'AGCCCAACTCCTCCTGAC3'
R55'GAGCAGCCTCATTCGGAC3'		 (TTGTG)8 55°C 3 0.441 0.613 0.223 280 bp–380 bp 31
TUGMS99 F5'GAATAGGGTTTGGCAGAGGC3'
R5'AGGATGGAGGAGGTGTCAA3'		 (GGGAGA)7
(GAGAA)6 		55°C 6 0.206 0.154 0.542 160 bp–200 bp 25
TUGMS102A F5'CGTAGCTCGCACACAACAC3'
R5'CGTCCCCTCCGAAATGA3'		 (GGAAA)12 55°C 6 0.706 0.803 0.230 100 bp–120 bp 27
TUGMS105 F5'GGGAGCTAGGGTTTTAGTTT3'
R5'CTTCAGAGCCACTTCTTTGTC3'		 (TTCTC)5 55°C 5 0.618 0.711 0.098 150 bp–200 bp 30
TUGMS108 F5'GGGACATCATCACCAGCTT3'
R5'TTCCTTGGTAGAACTCTGCTT3'		 (CAAAAA)6 55°C 6 0.853 0.790 0.141 130 bp–160 bp 30

TUGMS,Tea unigene microsatellite; bp, base pair; HO, Observed heterozygosities; HE, Expected heterozygosities)

* Accession details of contributing ESTs for unigenes are given in additional file (see Additional file 1).

**A significant deviation in Hardy-Weinberg equilibrium at P < 0.001 level recorded all single locus UGMS primers.

***TGUMS marker with multi locus amplifications.