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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Cell. 2008 Apr 4;133(1):103–115. doi: 10.1016/j.cell.2008.01.045

Figure 2. Cells with Low Levels of RanBP2 Form Chromosome Bridges in Anaphase.

Figure 2

(A) Chromosome dynamics and segregation of RanBP2+/+ and RanBP2−/H MEFs expressing H2B-YFP were observed by timelapse microscopy. Representative images of each mitotic stage are shown. Arrowheads mark chromatin bridges in RanBP2−/H MEFs. Bar = 10 μm.

(B) High-magnification images of RanBP2−/H MEFs with indicated chromosome segregation defects. Bar = 10 μm.

(C) Quantification of the chromosome segregation defects observed by live-cell imaging of MEFs of the indicated genotypes. n = total number of mitotic cells analyzed from at least three independent MEF lines. Error bars indicate SEM, *p < 0.05 versus wild-type cells (Chi-square test).

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