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FACS analysis of E. coli cells treated with FITC-labeled buforin II analogs. E. coli cells in midlogarithmic phase were washed with 10 mM NAPB and resuspended in the same buffer. E. coli cells (104 cfu) in 10 mM NAPB were incubated for 30 min at 25°C with 10 mM NAPB (control) (A), 16 μg/ml of FITC-BUF (5–21) (B), FITC-buforin II (C), FITC-BUF (8–21) (D), or FITC-BUF (5–20) (E).
