Table 2.
Antimicrobial activities of buforin II and its truncated analogs
| Microorganism | Minimal inhibitory concentration, μg/ml
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BUF II | BUF (5–21) | BUF (6–21) | BUF (7–21) | BUF (8–21) | BUF (9–21) | BUF (10–21) | BUF (11–21) | BUF (1–17) | BUF (5–20) | BUF (5–19) | BUF (5–18) | |
| Gram-positive bacteria | ||||||||||||
| Bacillus subtilis | 2 | 1 | 4 | 4 | 8 | 16 | 32 | 32 | >256 | 16 | 64 | 128 |
| Staphylococcus aureus | 4 | 2 | 8 | 8 | 16 | 64 | 32 | 64 | >256 | 64 | 128 | 256 |
| Streptococcus mutans | 2 | 1 | 4 | 4 | 8 | 32 | 32 | 32 | >256 | 32 | 64 | 256 |
| Streptococcus pneumoniae | 4 | 2 | 4 | 4 | 16 | 16 | 32 | 64 | >256 | 32 | 128 | 128 |
| Gram-negative bacteria | ||||||||||||
| Escherichia coli | 4 | 2 | 4 | 4 | 8 | 32 | 32 | 32 | >256 | 16 | 64 | 256 |
| Salmonella typhimurium | 1 | 1 | 2 | 2 | 4 | 16 | 16 | 32 | >256 | 16 | 32 | 128 |
| Serratia marcescens | 4 | 2 | 2 | 2 | 8 | 32 | 32 | 64 | >256 | 32 | 64 | 256 |
| Pseudomonas putida | 2 | 1 | 4 | 4 | 16 | 16 | 64 | 64 | >256 | 32 | 64 | 256 |
| Fungi | ||||||||||||
| Candida albicans | 1 | 1 | 8 | 8 | 32 | 64 | 32 | 64 | >256 | 64 | >256 | >256 |
| Cryptococcus neoformans | 1 | 1 | 8 | 8 | 64 | 64 | 64 | 128 | >256 | 64 | 128 | 256 |
| Saccharomyces cerevisiae | 1 | 1 | 8 | 8 | 32 | 64 | 64 | 128 | >256 | 128 | >256 | >256 |
Minimal inhibitory concentrations were the average values obtained in triplicates on three independent measurements.