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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Biochemistry. 2009 Mar 10;48(9):1975–1983. doi: 10.1021/bi802068s

Figure 2.

Figure 2

Representative ITC isotherms for the binding of bZIP domains of Jun-Fos heterodimer to dsDNA oligos containing the promoter sites TGACTCA (a), TTACTCA (b) and TGAGTCA (c). Note that the variant motif TGAGTCA is related to the wildtype TGACTCA motif by 2-fold symmetry. The position of the variant nucleotide in each of the sites relative to the consensus sequence TGACTCA is underlined. The solid lines represent the fit of the data points in the lower panels to a function based on the binding of a ligand to a macromolecule using the Microcal ORIGIN software (41).