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. Author manuscript; available in PMC: 2010 Mar 10.
Published in final edited form as: Biochemistry. 2009 Mar 10;48(9):1945–1953. doi: 10.1021/bi802254x

Table 2.

Summary of the spectral characteristics for recombinant wild-type and various mutants of the hTF N-lobe.

Protein (Iron bound) Color λmax (nm) A280/Amax
N-lobe (wild-type)a pink 472 23.5
G65R (HCO3−) yellow 426 21.8
G65R (HEPES) pink 474 28.2
G65R (Fe3+(NTA)2) pink 469 22.0
K206E orange 464 26.1
G65R/K206E pink 470 26.2
D63A (HCO3−)b yellow 414 21.2
H249A (HCO3−)c yellow 430 24.6
a

From He et al (8)

b

From He et al (7)

c

From He et al (10)