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Hydrogen-deuterium exchange reactions after 15 min at 37°C
| Protein | Increase in Mavga | % of Exchangea |
|---|---|---|
| Fe N-lobe (wild-type) | 112 ± 2 | 52 ± 1 |
| Apo N-lobe (wild-type) | 124 ± 2 | 59 ± 1 |
| G65R (HCO3−) | 129 ± 2 | 61 ± 1 |
| G65R (HEPES) | 126 ± 2 | 58 ± 1 |
| G65R (Fe3+(NTA)2) | 127 ± 2 | 58 ± 1 |
| G65R/K206E | 119 ± 2 | 55 ± 1 |
Measured value ± standard deviation
