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Bond lengths (Å) for the iron coordination in wild-type N-lobe and the two mutants, K206E and G65R/K206E
| Residue | N-lobe (wild-type) (1A8E) | K206E (2O84) | G65R/K206E (3FGS) |
|---|---|---|---|
| Asp63 OD1 | 2.03 | 2.20 | 2.04 |
| Tyr95 OH | 1.97 | 2.03 | 2.04 |
| Tyr188 OH | 1.80 | 2.31 | 2.20 |
| His249 NE2 | 2.04 | 2.58 | 2.23 |
| Arg124 carbonate | 2 conformations | 1 conformation | 1 conformation |
| Carbonate ligands NE NH2 |
2.81 2.57 |
2.93 2.90 |
2.67 2.64 |
