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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Methods. 2009 Feb 24;48(1):54–62. doi: 10.1016/j.ymeth.2009.02.012

Figure 2.

Figure 2

A. Schematic structure of the mutation reporter shuttle plasmid, pUCNIM. The non-B DNA-forming sequences (shown as “Non-B”) or the control sequence can be inserted between the lacZ gene and the promoter such that the function of the lacZ gene is maintained. B. RT-PCR analysis of dexamethasone-induced transcription through the non-B DNA-forming sequence (CG14) or control sequence in pUCG14 and pUCON plasmids. Primers were designed to bind sequences in the lacZ gene. RT-PCR products were subjected to agarose gel electrophoresis and visualized by ethidium bromide staining.