Skip to main content
. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Methods. 2009 Feb 24;48(1):54–62. doi: 10.1016/j.ymeth.2009.02.012

Figure 3.

Figure 3

The YAC breakage assay. Cells containing YAC CF1 (not to scale), which has a non-B DNA-forming sequence (shown as a red star) and URA3 gene, are FOAS. If breakage occurs in the region around the non-B DNA insertion, the YAC will lose the URA3 gene, resulting in FOAR. The YAC can be rescued by addition of a yeast telomere onto the C4A4 seed sequence. Adapted from Callahan et al (112).