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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: J Mol Biol. 2007 Oct 22;374(5):1145–1157. doi: 10.1016/j.jmb.2007.10.040

Table 1.

Split YFP Complementation by EED-EZH2 Interaction

Percentage of Positive Cells

Full-length 11.27 ± 0.33
Δ5′ 541 6.21 ± 0.52
Δ5′ 697 0.18 ± 0.04
Δ5′ 913 0.12 ± 0.02
Δ3′ 1663-End 0.28 ± 0.05
No transfect 0 ± 0

Legend: E14 embryonic stem cells were cotransfected with Venus 1-EZH2 and EED-Venus 2 expression constructs. 24 hours post-transfection, triplicate samples were analyzed by FACS. The mean percentage of YFP-positive cells and corresponding standard deviation from a representative experiment are shown.