Table 1.
Split YFP Complementation by EED-EZH2 Interaction
| Percentage of Positive Cells | |
|---|---|
| Full-length | 11.27 ± 0.33 |
| Δ5′ 541 | 6.21 ± 0.52 |
| Δ5′ 697 | 0.18 ± 0.04 |
| Δ5′ 913 | 0.12 ± 0.02 |
| Δ3′ 1663-End | 0.28 ± 0.05 |
| No transfect | 0 ± 0 |
Legend: E14 embryonic stem cells were cotransfected with Venus 1-EZH2 and EED-Venus 2 expression constructs. 24 hours post-transfection, triplicate samples were analyzed by FACS. The mean percentage of YFP-positive cells and corresponding standard deviation from a representative experiment are shown.