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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Mol Cell Biochem. 2009 Feb 15;327(1-2):111–126. doi: 10.1007/s11010-009-0049-x

Fig. 5. rGPx-3 and Plasma GPx-3 Forms.

Fig. 5

Western blot of His-Gravi Trap purified rGPx-3 in Cos7 media were run on a SDS-PAGE gel under reducing and non-reducing conditions and compared to partially purified GPx-3 from human plasma. a) rGPx-3 was detected using an anti-V5 antibody; b) GPx-3 from human plasma was detected by using a monoclonal GPx-3 antibody; and c) densitometric ratio of tetrameric-to-monomeric forms of rGPx-3 and partially purified GPx-3 from plasma. The Western blot is representative of three separate experiments. Each bar represents the average densitometry of three experiments, with values given as mean +/− SD.