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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Dev Biol. 2007 Aug 31;311(2):396–407. doi: 10.1016/j.ydbio.2007.08.046

Figure 2.

Figure 2

The temporal and spatial expression pattern of Lef1, Tcf3, and Tcf4 in the developing pituitary gland. In situ hybridization was used to analyze Lef1, Tcf3, Tcf4, and Tcf4E expression in the developing anterior pituitary gland and the adjacent dorsal, caudal domain (cd) and ventral, rostral domain (rd) of the ventral diencephalon. Immunohistochemistry utilizing an antibody that recognizes all TCF4 isoforms was used to confirm developmental expression. The scale bar in panel U represents 50μm and should be applied to e10.5 (A, F, K, P, and, U), and e11.5 (B, G, L, O, Q, T, V, and Y) pituitary sections. The scale bar for the e12.5 (B, H, J, M, R, and V), e13.5 (N and X) and e14.5 (C and I) pituitary sections represents 100μm and is shown in panel W. The arrow in e12.5 sections labeled with Tcf4E marks the boundary between the caudal and rostral domains of the ventral diencephalon. The insets in panels L and V represent sense probes from Tcf4 and Tcf4E, respectively. Panel S is a high magnification (400X) image of an adult pituitary gland.