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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Cell Neurosci. 2009 Apr 1;41(2):274–285. doi: 10.1016/j.mcn.2009.03.007

Figure 4. Rac1-dependent actin filament reorganization requires redox intermediates.

Figure 4

SH-SY5Y human neuroblastoma cells grown on collagen were transfected (lipofectamine) with FLAG-V12Rac1. One day post-transfection, V12Rac1-expressing SH-SY5Y cultures and non-transfected cultures were treated with 10 μM DPI (30 min) or 40 μM MnTBAP (1 h) and then fixed with paraformaldehyde. Cultures were then stained for actin filaments (rhodamine phalloidin, upper panel) FLAG-V12Rac1 (anti FLAG/fluorescein, middle panel). Representative confocal images of SH-SY5Y cells were acquired and image overlaid revealed colocalization of actin filaments and V12Rac1 expression (lower panel, yellow). Expression of V12Rac1 stimulated dramatic lamellipodia formation and spreading of SH-SY5Y cells. In contrast, both incubation with DPI or MnTBAP negated lamellipodia formation and cell spreading with cell morphologies similar to non-transfected cells. V12Rac1 was predominantly localized to the cell periphery. (Scale bar = 10 μm)