. Author manuscript; available in PMC: 2010 Jun 1.

Published in final edited form as: Mol Cell Neurosci. 2009 Apr 1;41(2):274–285. doi: 10.1016/j.mcn.2009.03.007

Table I.

TNFα and Il-1β elicit a transient formation of lamellipodia and membrane ruffles.

Exposure	% active cells
Exposure	15 min	30 min
PBS	15±4 %	12±4%
20 ng/ml TNFα	43±4 % ^*	7±4 %
50 ng/ml TNFα	75±5 % ^*	3±4 %
200 ng/ml TNFα	80±4 % ^*	10±3 %
20 ng/ml Il-1β	48±3 % ^*	8±4 %
50 ng/ml Il-1β	53±3 % ^*	10±4 %
200 ng/ml Il-1β	83±3 % ^*	7±4 %

A total of at least 180 randomly chosen SH-SY5Y human neuroblastoma cells per condition were analyzed from at least three independent experiments. Cultures were scored for active cells according to our criterion; SH-SY5Y cells displaying at least two distinct regions with lamellipodia formation and/or membrane ruffling. Cytokines (200 ng/ml) and PBS were added to serum-free culture medium as volumes accounting for 2% or less of the total culture medium.

Increase in the percentage of cells responding with formation of lamellipodia and membrane ruffling that is significantly different from control (p<0.0001).