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Significance of the RBS region for S. aureus reporter expression. A. Graphic map of the pAH14-pAH17 plasmids and the different RBS sequences used. B. S. aureus RN4220 was transformed with each of the four plasmids, and cultures were grown for 4.5 hr (log phase) and 7 hr (stationary phase). YFP10B fluorescence was measured in a plate reader and readings were plotted relative to cell density at 595 nm.
