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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Microbiol Methods. 2009 Mar 3;77(3):251–260. doi: 10.1016/j.mimet.2009.02.011

Figure 9.

Figure 9

Flow sorting of S. aureus labeled with fluorescent reporters. SH1000 without a plasmid served as a negative gating control (histograms A & B). Plasmids pAH1 (agr P3, sarA RBS, mCherry) and pAH16 (sarA P1, sod RBS, YFP10B) were used for the FACS test. SH1000 (agr+) was transformed with pAH1 and pAH16, resulting in strain AH1218 (histograms C & D). Similarly, SH1001 (Δagr) was transformed with the same plasmids, resulting in strain AH1219 (histograms E & F). Each strain was grown overnight in TSB (with Cam and Erm as needed), cell density was adjusted to 106 CFU/mL, and populations were separated by FACS. Histograms A, C, and E display the yellow fluorescence channel and B, D, F display the red fluorescence channel. Units represent relative cell counts detected by the flow cytometer plotted at the measured fluorescence intensity.