SDS/PAGE. Lanes: A, molecular weight markers; B, crude cell extract of the recombinant E. coli strain XL1-pNCO-HIS6-TM-YCHB; C–E, recombinant catalytic domain of 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase from tomato after chromatography by affinity, anion exchange, and size exclusion, respectively.