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Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic | Reference or source |
|---|---|---|
| E. coli | ||
| XL1-Blue | RecA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac, [F′, proAB, lacqZΔM15, Tn10 (ter)] | Ref. 28, Stratagene |
| Plasmids | ||
| pACYC184 | Cloning vector | Ref. 27, New England Biolabs |
| pNCO113 | High copy expression vector | ATCC, PTA-852 |
| pNCO-SB-HIS6-ACYC184 | High copy His-tag expression vector | This study |
| pNCO-HIS6-TM-YCHB | This study |
