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Purification of recombinant 4-disphosphocytidyl-2-C-methyl-d-erythritol kinase from tomato
| Step | Total protein, mg | Total activity, μmol⋅min−1 | Specific activity, μmol⋅mg−1⋅min−1 | Yield, % | Purification, fold |
|---|---|---|---|---|---|
| Cell extract | 488 | 683 | 1.4 | 100 | 1 |
| Ni-Sepharose | 17 | 323 | 19 | 47 | 14 |
| Mono Q | 6.4 | 198 | 31 | 29 | 22 |
| Superdex 200 | 1.6 | 53 | 33 | 8 | 24 |
