Figure 4.
A) Modified image from Paxinos and Watson (1998) 3.3 mm caudal to bregma showing 1) The area depicted in images B–G, 2) The area measured for intensity of staining for the amygdala. Image B, C and D are representative images from group housed rats showing: B) CB1 expression, C) FAAH expression and D) D2 expression. Image E, F and G are representative images from socially-isolated rats showing: E) CB1 expression, F) FAAH expression and G) D2 expression and H) Effect of social isolation on CB1, FAAH and D2 relative fluorescence density of staining in the amygdala compared with group housed rats. * denotes P < 0.05 when compared with group housed rats (n = 7).