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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: Vaccine. 2009 Feb 5;27(25-26):3359–3366. doi: 10.1016/j.vaccine.2009.01.079

Table 1.

Optimal cell culture conditions for infection of PBMC for detection of rubella-specific cytokine secretion.

Cytokine Rubella virus MOI Length in culture
IL-2 5 8 days
IL-4, IL-5 5 24 h
IL-6, IL-10 5 24 h
IL-12p40, GM-CSF 5 18 h
TNF-α 0.05 8 days
IFN-γ 5 2 days

MOI—multiplicity of infection.