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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: FEBS Lett. 2007 Jul 25;581(21):3967–3972. doi: 10.1016/j.febslet.2007.07.037

Fig. 1.

Fig. 1

Presence of ALDH1 protein in the oxidized cytosolic proteins after ethanol exposure. Oxidized cytosolic proteins from dextrose-treated controls (−) or ethanol-exposed (+) rat livers were labeled with biotin-NM. Biotin-NM labeled proteins (20 μg/well) were then separated on 12% SDS-PAGE, transferred to PVDF-Immobilon membranes, and stained with Coomassie blue (A) or subjected to immunoblot analysis using streptavidin-HRP antibodies (B). Presence of ALDH1 protein in oxidized cytosolic proteins was confirmed by immunoblot analysis. Biotin-NM-labeled cytosolic proteins before (C) or after (D) purification with streptavidin-agarose were analyzed by 1D SDS-PAGE, and then subjected to immunoblot analysis (IB) using the specific anti-ALDH1 antibody. This figure represents a typical result from at least two different experiments.