Skip to main content
. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Chem Biol. 2007 Dec;14(12):1323–1333. doi: 10.1016/j.chembiol.2007.10.016

Figure 6.

Figure 6

Angiogenic activity of Y341A TyrRS not from serendipitous proteolytic activation. (A) Less mini form of Y341A TyrRS than of TyrRS was generated in the media during the endothelial cell migration assay, as shown by western blot analysis with polyclonal antibody against TyrRS. This observation suggested that the cytokine activation of TyrRS by the Y341A mutation was not a result of proteolysis. (B) Time courses of plasmin and elastase digestions of both wild-type and the Y341A TyrRS. The Y341A TyrRS was as stable as the wild-type enzyme during the digestion. Arrows point to the mini form of TyrRS.