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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Anal Biochem. 2007 Dec 14;374(2):304–312. doi: 10.1016/j.ab.2007.12.008

Table 1.

Equilibrium association constants (Kobs) and free energy (ΔG°) for site-specific binding of BrD3 with the five acetylated histone H3 peptides

Temperature
(K)		 Na+
(M)		 AcK4
 AcK9
 AcK14
 AcK18
 AcK23
Kobs
(105 M-1)		 ΔG
(kcal/mol)		 Kobs
(105 M-1)		 ΔG
(kcal/mol)		 Kobs
(105 M-1)		 ΔG
(kcal/mol)		 Kobs
(105 M-1)		 ΔG
(kcal/mol)		 Kobs
(105 M-1)		 ΔG
(kcal/mol)
283 0.10 0.3 -5.6 4.6 -7.3 0.3 -5.9 0.7 -6.3 0.6 -6.2
288 0.10 0.3 -5.9 7.4 -7.7 0.3 -5.9 0.8 -6.4 1.0 -6.6
298 0.10 0.4 -6.3 14.1 -8.4 0.4 -6.2 1.0 -6.8 1.1 -6.9
303 0.10 0.5 -6.5 11.1 -8.4 0.4 -6.4 0.8 -6.8 1.0 -6.9
308 0.10 0.4 -6.5 5.2 -8.1 0.3 -6.3 0.6 -7.1 0.7 -6.9
313 0.10 0.3 -6.2 2.9 -7.8 0.2 -6.3 0.6 -6.4 0.4 -6.6
298 0.05 0.4 -6.3 13.7 -8.2 0.3 -6.1 1.1 -6.9 1.1 -6.9
298 0.15 0.4 -6.3 13.9 -8.4 0.4 -6.2 0.9 -6.8 1.1 -6.9
298 0.20 0.4 -6.3 10.4 -8.2 0.2 -5.9 0.8 -6.7 1.0 -6.8
298 0.25 0.3 -6.2 8.3 -8.1 0.3 -6.1 0.5 -6.4 1.0 -6.8