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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Feb 13;381(2):229–235. doi: 10.1016/j.bbrc.2009.02.029

Figure 1.

Figure 1

Figure 1

Figure 1

Activin A and inhibin A regulate early thymocyte development. E14 FTOC were performed for three days with 500 ng/ml of activin A or inhibin A and analyzed by flow cytometry. A, thymocytes were stained with anti CD25-FITC and anti CD44-PE coupled antibodies to differentiate between DN1, DN2, DN3, and DN4 subpopulations. Data represents percentage of DN thymocytes. B, Thymocytes stained with anti CD4-PE and anti CD8-Cy coupled antibodies. Data represents percentage of DN, DP, CD4 and CD8 thymocyte subpopulations. The average (−) of ten individual mice from at least four independent experiments is shown. ** Statistical differences (p ≤ 0.05) using paired T-test between control and treated lobes. C, Representative dot plot of thymic precursors from day 3 FTOCs with or without 500 ng/ml of activin A. Thymocytes were stained with anti CD4-APC, anti CD8-Cy, anti CD24-FITC and anti TCRβ-PE. Histograms represent CD24 and TCRβ expression of FTOC-derived and adult thymus CD8+ gated cells. Data obtained from a representative mouse (n= 10), from two independent experiments.