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SDS-PAGE analysis of highly enriched recombinant PAI-2 proteins. 10 μg of total protein from PAI-2 ΔCD-loop purified from either pET15b (lane 1) or pQE9 (lane 2), or wild-type PAI-2 from pQE9 (lane 3) were fractionated by a 10% SDS-PAGE under reducing conditions. The slight difference in size between PAI-2 ΔCD-loop from pET15b and pQE9 is due to differences in tag/linker length. * marks cleaved PAI-2 in wild-type population as mentioned in text.
