Table 1.
Kinetic parameters of the interaction between PAI-2 ΔCD-loop or wild-type PAI-2 and VLDLR, both alone and in complex with uPA.
| Analyte | Binding Model |
ka (M-1 s-1) |
kd (s-1) |
KD (nM) |
χ2 |
| PAI-2 wild-type | No binding | - | - | - | - |
| uPA:PAI-2 wild-type | 1:1 | 3.75 × 105 (± 1.1 × 105) |
1.44 × 10-3 (± 0.39 × 10-3) |
4.81 (± 0.64) |
4.71 |
| PAI-2 ΔCD-loop | No binding | - | - | - | - |
| uPA:PAI-2 ΔCD-loop | 1:1 | 4.16 × 105 (± 1.8 × 105) |
1.36 × 10-3 (± 0.23 × 10-3) |
4.34 (± 0.56) |
3.28 |
Values were determined using surface plasmon resonance. Binding data was fitted using the BIAevaluation 4.0 software. The binding model chosen represents that with the lowest χ2 value. (Values are the average ± SEM, n = 3).