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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Proteome Res. 2009 Mar;8(3):1285–1292. doi: 10.1021/pr8006107

Table 1.

Prediction of relative standard deviation for a multiple-step workflow based on measured errors contributed by each step.

N SI SF SD SQ S
1 0.152 0.197 0.046 0.080 0.265
3 0.089 0.067 0.037 0.080 0.142
6 0.072 0.037 0.000 0.080 0.114

N: replicate number; SI, SF, SD, and SQ: the RSDs contributed by IP, fractionation, digestion, and SILAC ratio measurement by mass spectrometry/MSQuant respectively; S: the predicted RSD for the entire workflow using N replicates of IP, fractionation and digestion.