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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: J Proteome Res. 2009 Mar 6;8(3):1271–1284. doi: 10.1021/pr800601x

Figure 2.

Figure 2

Outline of method used to enrich for labeled populations of neurons in the adult D. melanogaster brain. Adult brains genetically labeled with the surface marker mCD8-GFP using the GAL4-UAS expression system are dissociated into single cell preparations and incubated with paramagnetic beads conjugated to a monoclonal anti-mCD8 antibody. The mixture is placed in a magnetic column allowing antibody-captured cells to be retained in the column. When the magnet is removed, the cells are eluted. Following an acidified methanol extraction, the contents of the immunoaffinity-purified cells are analyzed by MALDI-TOF mass spectrometry.