Table 1.

Summary of Amino Acid Sequences of the Peptides Identified in This Study (Using LC-ESI-QTOF-MS/MS and/or Exact Mass Measurements Employing Off-line HPLC MALDI-FTMS) and Previously Identified Peptides

Encoding genea	Peptide Family	Neuropeptide Sequenceb	Previous work			This work
			Larval Tissuec,d		Adult Tissuee	Adult Tissue
			1-D LC/MS	2-D LC/MS	Direct MALDI MS	ESO-Q-TOF MS	MALDI-FTMS
CG1171f	Adipokinetic hormone (Akh)	pQL TFSPDWa	+		+
		c.pQL TFSPDWGK.R			+	+
CG 13633g Y/F(X)FGL-mide	Allatostatin A(Ast)	KR.LPVYNFGLa.GKR				+
		KR.SRPYSFGLa.GKR	+	+	+
		KR.VERYAFGLa.GRR			+
		RR.AYMYTNGGPGM.KR	+
		KR.TTRPQPFNFGLa.GRR	+	+	+	+	+
CG6456h W(X)6Wa	Allatostatin B (Myoinhibiting peptide)	KR.AWKSMNVAWa.GKR	+	+
		KR.DQWQKLHGGWa.GKR	+	+			+
		KR.RQAQGWNKFRGAWa.GKR			+	+	+
CG14919i	Allatostain C(Ast C)	L.FAQYRPTSYSA.Y				+
		A.YLRSPTYGNVNEL.Y				+
CG3302j	Corazonin(Crz)	G.pQTFQYSRGWTNa.GKR	+	+	+		+
CG6440k	Dromyosupressin (Dms)	KR.TDVDHVFLRFa.GKR	+	+	+	+	+
		KRT.DVDHVFLRFa.GKR		+
		KR.TDVDHVFLR.FGKR				+
CG18090l	Drosulfakinin (Dsk)	KR.FDDYGHMRFa.GKR		+	+	+
		KR.FDDYGHmRFa.GKR				+
		KR.GGDDQFDDYGHMRFa.GR	+		+	+
CG2346m	FMRFamide	KR.AAMDRYa.GR				+
		R.SDNFMRFa.GR	+	+	+
		R.PDNFMRFa.GR	+	+	+
		R.SAPQDFVRSa.GK			+	+
		GK.MDSNFIRFa.GK		+	+
		R.TPAEDFMRFa.GR	+	+	+	+
		RR.SVQDNFMHFa.GKR		+
		R.SPKQDFMRFa.GR			+
		R.DPKQDFMRFa.GR	+	+	+	+
		RD.PKQDFMRFa.GR				+
CG6371n	HUGIN	KK.SVPFKPRLa.GKR	+	+	+	+
CG13480o	Leucokinin (Drm-kinin)	KR.NSVVLGKKQRFHSWGa.GKR	+	+	+	+
CG13968p	Short Neuropeptide Fs (short NPFs)	RK.PQRLRWa.GR				+
		R.SPSLRLRFa.GR		+	+	+	+
		R.KPMRLRWa.GR				+
		KR.WFGDVNQKPHR	+	+		+	+
		R.SDPDMLNSIVE.KR	+	+	+	+
		R.SDPDmLNSIVE.KR	+
		RK.AQRSPSLRLRFa.GR			+
CG3441q	Neuropeptide-like precursor 1 (Nplp 1)	KR.SVAALAAQGLLNAPK.R	+	+	+	+	+
		KR.YIGSLARAGGLMTYa.GKR	+	+	+	+
		KR.NLGALKSSPVHGVQQ.KR		+		+
		KR.NVGTLARDFQLPIPNa.GKR	+	+	+	+	+
		KR.NLGALKSSPVHGVQ.QKR				+
		KR.YIGSLARAGGLMT.YGKR				+
CG11051r	Neuropeptide-like precursor 2 (Nplp2)	L.TKAQGDFNEF.I	+
		R.EESNPAQEFLTK.A		+
		K.AQGDFNEFIEKLK.A		+
		R.EESNPAQEFLTKAQGDF.N				+
		R.EESNPAQEFLTKAQGDFNEF.I				+
		F.LTKAQGDFNEF.I				+
CG6496s	Pigment dispersing factor (PDF)	KR.NSELINSLLSLPKNMNDAa.GK				+
CG15520t F(X)PPLamide or PRVamide	Pyrokinin (Capa)	KK.ASGLVAFPRVa.GR	+	+	+
		RR.GANMGLYAFPRVa.GR	+	+	+
		KRT.GPSASSGLWFGPRLa.GKR		+	+	+	+
		KR.TGPSASSGLWFGPRLa.GKR		+	+	+	+
CG33527u	SIFamide	A.AYRKPPFNGSIFa.GKR				+	+
		AA.YRKPPFNGSIFa.GKR		+	+	+	+
CG14734v F(X)1G(X)2Ramide	Tachykinin-like peptides	KR.APTGFTGMRa.GKR		+
		KK.APLAFVGLRa.GK		+
		KR.APNGFLGMRa.GKR		+		+	+
		KR.APTSSFIGMRa.GKK		+	+
		KR.APVNSFVGMRa.GKK		+	+	+	+
CG15231w	Immune-induced peptide (IM4)	P.GTVLIQTDNTQYIRTa.G.	+			+

^aDrosophila computated gene (CG) number.

^bAmino acid sequences of peptides flanked by the predicted cleavage sites (indicated by •). Fields in yellow indicate previously unidentified peptides. Fields in blue indicate precursors or fragments of identified peptides. Fields in gray indicate peptides not predicted to be flanked by mono- or dibasic cleavage sites according to the encoding gene.

^cOne-dimensional LC/MS on extract from larval nervous system (ref. ²⁰).

^dTwo-dimensional LC/MS on extract from larval nervous system (ref. ¹⁹).

^ePeptide expression characterized by direct MALDI-TOF MS analysis on dissected tissue (refs ²¹, ²²).

^fRefs ³³, ³⁴.

^gRef ³⁷.

^hRef ³⁹.

ⁱRef ⁴⁰.

^jRef ⁴².

^kRef ⁴⁸.

^lRef ⁵¹.

^mRefs ⁵⁵, ⁵⁶.

ⁿRef ⁵⁸.

^oRef ⁶³.

^pRefs ³⁰, ⁶⁶.

^qRef ²⁰.

^rRef ²⁰.

^sRef ⁷¹.

^tRefs ¹⁰⁶, ¹⁰⁷.

^uRef ⁷⁶.

^vRef ⁷⁹.

^wRef ⁸⁰. m, oxidized Met residue; q, pyroglutamic acid; X, any amino acid.