. Author manuscript; available in PMC: 2010 Jul 15.

Published in final edited form as: Exp Cell Res. 2009 Apr 14;315(12):2001–2011. doi: 10.1016/j.yexcr.2009.04.003

Table 2.

Shank3 recruitment to EPEC attachment is Tir-dependant

	Bacteria/cell	Pedestals/100 bacteria	Shank3-staining/100 bacteria
ΔTir	3.2 (+/− 1.1)	0.0 (+/− 0.0)	0.2 (+/− 0.3)
pTir	7.3 (+/− 0.9)	73.1 (+/− 3.1)	41.3 (+/− 2.2)
Y454F	8.2 (+/− 1.4)	68.2 (+/− 2.3)	35.1 (+/− 3.1)
Y474F	2.2 (+/− 1.2)	4.1 (+/− 3.2)	0.2 (+/− 0.5)
Y454F/Y474F	1.8 (+/− 1.3)	0.3 (+/− 0.4)	0.1 (+/− 0.2)

HeLa cells were transfected with Shank3-HA and infected 24 hours later. Infections were performed using EPEC expressing wild-type or mutant Tir variants for 5 hours, cells were then fixed and stained for bacteria, actin and Shank3. Shank3 staining was performed using anti-Shank3 antibodies since bacterial Tir expression constructs were HA-fusions. Quantification was performed by a blinded operator and data was pooled from two independent experiments and is presented as means with standard deviations.