. 2009 May 13;6:44. doi: 10.1186/1742-4690-6-44

Table 4.

Formation of DEC or DEC-like complex by ddNTP-terminated DNA and HIV-1 RT in the presence of next correct dNTP, ddNTP or EFV.

Dideoxyadenosine analog as the chain-terminator
			Next Correct dNTP or ddNTP						EFV

DNA	Terminator		dCTP			FTC-TP

		K_d(μM)	B_max(%)	B_max/K_d	K_d(μM)	B_max(%)	B_max/K_d	K_d(μM)	B_max(%)	B_max/K_d

D20/D36	TFV	232 ± 12^a	18.5 ± 6.7	0.079	250 ± 11	17.7 ± 3.9	0.071	0.70 ± 0.10	45.7 ± 10.0	65
	ddAMP	21.5 ± 9.8	34.2 ± 4.6	1.6	171 ± 28	28.1 ± 4.7	0.16	1.20 ± 0.38	16.3 ± 3.5	13.6

D26/D50	TFV	179 ± 33	50.0 ± 5.5	0.28	46.5 ± 7.8	50.0 ± 5.0	1.1	1.22 ± 0.28	44.4 ± 3.7	36
	ddAMP	8.1 ± 2.1	55.8 ± 3.4	6.9	86.8 ± 0.2	60.2 ± 7.2	0.69	2.42 ± 1.10	11.2 ± 0.6	4.6

Dideoxycytidine analog as the chain-terminator

			Next Correct dNTP or ddNTP						EFV

DNA	Terminator		dATP			TFV-DP

		K_d(μM)	B_max(%)	B_max/K_d	K_d(μM)	B_max(%)	B_max/K_d	K_d(μM)	B_max(%)	B_max/K_d

D20/D36	FTC-MP	ND^a	ND	ND	ND	ND	ND	1.54 ± 0.33	5.53 ± 1.83	3.0
	ddCMP	16.9 ± 8.5	38.0 ± 4.4	2.2	445 ± 114	15.3 ± 2.7	0.034	1.03 ± 0.25	14.9 ± 0.8	14.5

D27/D50	FTC-MP	862 ± 229	45 ± 14	0.052	ND	ND	ND	1.26 ± 0.24	3.96 ± 1.55	3.1
	ddCMP	6.5 ± 2.6	60 ± 3	9.2	140 ± 19	55 ± 2	0.39	0.11 ± 0.06	10.6 ± 2.0	96

All Values reported are average ± standard deviation from at least three measurements.

^aND = No DEC formation was detected at the highest concentration (2 mM) tested.