Figure 6. Saturation curve for recDpl with increasing α₂M concentrations.

The working range of coated-α₂M was from 25 ng to 1.6 µg/well. recDpl was added 1.0 µg/well. A trend of linearity is observed for values up to 100 ng/well, whereas a trend of plateau can be found for values higher than 400 ng/well. No difference is observed in the binding to recDpl comparing the native form with the activated form of α₂M. y axis, OD values at 405 nm (x1,000); x axis, µg of proteins per well; white squares, α₂M “fast” form; black rhombi, α₂M “native” form.