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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Curr Biol. 2008 Feb 12;18(3):211–215. doi: 10.1016/j.cub.2008.01.007

Figure 3. Arr4 is a GEF for Gpa1.

Figure 3

(A) and (B), Single turnover GTP binding assay. Time-course of [35S]GTPγS binding to 100 nM purified 6xHIS-Gpa1 in the presence of 200 nM GST-Arr4. Results are the mean ± SEM of duplicate samples, and are presented as percent of maximum bound (saturated binding occurred between 50-75%).

(A) with 500 nM CuSO4 added.

(B) Same as in A, but no copper added to the reaction.

(C) Steady state GTP hydrolysis assay. Time-course of Pi released in the presence or absence of 6xHIS-Gpa1 (250 nM), GST-Arr4 (500 nM), and copper (500 nM). Results are the mean ± SEM of duplicate samples.