Fig. 3a,b.

Effects of aging and CR on carnitine palmitolytransferase-1 (CPT-1) activity in skeletal muscle. a Mitochondria were extracted and CPT-1 activity was assessed using spectrophotometry at 324 nm. b RT-PCR analysis of CPT-1 was performed on RNA isolated from AL and CR rats. β-actin primers were used in separate PCR reactions to control for the efficiency of cDNA synthesis in each sample. One representative result is shown from three experiments that yielded similar results. * P < 0.05 compared with 5-month-old AL rats, ## P < 0.01 compared with each CR rat, respectively