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. 2000 Jul 4;97(15):8278–8283. doi: 10.1073/pnas.140213797

Figure 1.

Figure 1

RNA fragments that appeared in response to the action of colicin D. (A) RNAs were prepared from W3110 or DR1 cells grown in 100 ml of medium and then incubated with or without partially purified colicin D. The RNAs (20 μg) were separated on a 10% polyacrylamide gel containing 7 M urea in TBE buffer (16) and then were stained with ethidium bromide. RNA1 and RNA2, which appeared specifically with colicin-treated W3110, are indicated by arrowheads. Direct sequencing according to Donis-Keller (21) showed that 5′-labeled RNA1 corresponded to the 3′-fragment (positions 39–76) of tRNAArgICG (B) and that 3′-labeled RNA2 corresponded to the 5′-fragment (positions 1–34) of tRNAArgICG (C). -, OH, T1, U2, Ph, and CL indicate samples without digestion, and digested with alkali, and RNasesT1, U2, PhyM, and CL3, respectively.