Fig. 1.

Netrin inhibits the regenerative neurite growth of adult DRG neurons in cultures. (A) Representative images of the control explant (left) and netrin-1 (500 ng/mL) incubated explant (right). (B) Measurement of neurite outgrowth from anti-Tuj1 stained explants. Values shown are the means±s.d. from three independent experiments, and 20-25 explants were counted for each experiment. (C) Representative images of the control (left) and netrin-1 (500 ng/ mL)-treated dissociated neurons (right). (D, E) Measurement of neurite-bearing neurons (D) and length of the longest neurite from 180-200 dissociated neurons for each measurement (E). Values shown are the means±s.d. from three independent experiments (^*p<0.05, Student's t-test). Bars in A and C, 100 µm.