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Expression of snail transcription factor and the loss of E-cadherin expression. A549 cells were cultured for 48 hr without TGF-β1 (A), with 0.5 ng/mL TGF-β1 (B), and in a combination of 0.5 ng/mL TGF-β1 plus 2 ng/mL IL-1β (C). 0.5 ng/mL TGF-β1 began to reveal loss of E-cadherin expression and increasing snail expression, and addition with IL-1β shows the elevation of snail expression.
