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. 2009 Jun 5;10(7):776–782. doi: 10.1038/embor.2009.88

Figure 2.

Figure 2

MC1568 stabilize the MEF2–HDAC4–HDAC3 complex and blocks myogenesis of C2C12 cells. (A) Western blot of myogenin and αMHC in the absence and presence of differentiation medium (DM) with or without MC1568 added to the DM at the start of differentiation (lane 3) or after 24 h (lane 4); HSP70 indicates equal loading. (B) Western blot of MEF2D and HDAC4 expression in C2C12 cells; α-tubulin (Tub) indicates equal loading. (C) Immunoprecipitation (IP) assays using MEF2D antibodies for MEF2D IPs and HDAC4 co-IPs in C2C12 cells. Acetyl-MEF2D (AcMEF2D) levels were revealed with antibodies against acetylated lysines. (D) IPs using MEF2D and HDAC3 antibodies to show MEF2D, HDAC3 and HDAC4 complexes in differentiating C2C12 cells with or without treatment with MC1568; note that IgG-negative control IP did not show detectable bands. HDAC, histone deacetylase; HSP70, heat-shock protein 70; MEF2, myocyte enhancer factor 2; αMHC, α-myosin heavy chain.