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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Cell Metab. 2007 Sep;6(3):181–194. doi: 10.1016/j.cmet.2007.08.003

Figure 6.

Figure 6

Elevated protein turnover in vivo and mTOR signaling in BCATm null mice

A) In vivo protein synthesis rates measured by flooding does of L-[3H]phenylalanine. ** P < 0.01, n=10–13.

B) Amount of 24-h urinary 3-methyhistidine and molar ratio of urine 3-methyhistidine to creatinine. Eight male mice from each genotype were placed in individual metabolic cages (Nalegene, Rochester, NY) for 2 days. Daily urine was collected for analysis of 3-methyhistidine and creatinine. ** P < 0.01 and *** P < 0.001, n=16.

C) Western blot analysis for 4E-BP1 and pT37/46 4E-BP1 in gastrocnemius of BCATm−/− mice fed a choice of NC/−BCAA diets. ** P < 0.01, n=8.

D) Western blot analysis for 4E-BP1, pS235/236 S6, S6, pT389 S6K1, and S6K1 in gastrocnemius of fasted (F) and fasted-refed (R) BCATm−/− mice. * P<0.05, ** P < 0.01 and *** P < 0.001, n=4 for fasted and 8 for fasted-refed.