Skip to main content
. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: J Biol Chem. 2006 Nov 27;282(5):2871–2879. doi: 10.1074/jbc.M608083200

FIGURE 7. ATP-dependent ROS production and ERK1/2 activation are upstream from caspase-1 activation.

FIGURE 7

A, macrophages were stained with the fluorescent caspase substrate, FAM-VAD-fmk, pretreated for 10 min with the redox inhibitor, DPI (2 µm), and then incubated for 6.5 or 12 h with 3 mm ATP. Caspase-1 was measured by FACS. The specificity of caspase-1 activation was verified by pretreating the stained cells with the irreversible caspase-1 inhibitor, Z-YVAD-fmk. p < 0.001 for cells treated with DPI and ATP for 12 h, compared with cells treated with ATP for 12 h. B, stained macrophages were pretreated for 5 min with 10 µm of the ERK1/2 inhibitor, PD98056, for 5 min, before incubation for 5 min with 3 mm ATP. p < 0.001 for cells treated with PD98056 and ATP for 6.5 or 12 h, compared with cells treated with ATP alone. The values show averages and S.D. from experiments performed in triplicate, and represent results obtained from at least two representative experiments.