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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: J Biol Chem. 2006 Nov 27;282(5):2871–2879. doi: 10.1074/jbc.M608083200

FIGURE 8. ATP stimulation of macrophages leads to IL-1β secretion through a pathway requiring ROS production and PI3K activation.

FIGURE 8

Macrophages were primed with 1 µg/ml LPS for 2 h at 37 °C, before treating the macrophages with the caspase-1 inhibitor, Z-YVAD-fmk (50 µm) for 30 min, DPI (2 µm) for 10 min, or LY294002 (50 µm) for 10 min. The cells were then stimulated with 3 mm ATP for 6 h. Secretion of IL-1β was measured by ELISA. p < 0.001 for cells treated with ATP and Z-YVAD-fmk, DPI, or LY294002, compared with cells treated with ATP alone. The experiment was performed twice in duplicate, and the results represent the average and S.D.