Figure 2. EGFP labeling at E12.5 in I12b-Cre;Z/EG and URE2-Cre;Z/EG reveals strong forebrain expression.
(A-B) Immunohistochemistry for EGFP on parasagittal sections through an E12.5 (A) I12b-Cre;Z/EG or (B) URE2-Cre;Z/EG animal.
(C-D) Immunohistochemistry for EGFP on coronal sections through the E12.5 forebrain of (C) I12b-Cre;Z/EG or (D) URE2-Cre;Z/EG mice. EGFP expression is present in the CGE in I12b-Cre;Z/EG but not URE2-Cre;Z/EG. Also note that tangentially migrating cells (arrowhead) are readily detected in I12b-Cre;Z/EG forebrain.
cge, caudal ganglionic eminence; drg, dorsal root ganglion; ge, ganglionic eminences; hyp, hypothalamus; lge, lateral ganglionic eminence; mge, medial ganglionic eminence; ob, olfactory bulb; oe, olfactory epithelium; poa, preoptic area; pth, prethalamus; s, skin; svz, subventricular zones; v, trigeminal ganglion. Scale bar in A = 1mm and in C = 500 μm.