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. 2009 Jun 17;4(6):e5926. doi: 10.1371/journal.pone.0005926

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Legrand et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence and confocal microscopy (insets) analysis of γδTCR/CD3 chains expression on cytospin eosinophil preparations after 18 h culture. Staining with anti-αTCR, anti-βTCR antibodies and control goat IgG is represented. Arrowheads indicate some positive cells. (B) Surface of eosinophils and γδT cells was biotin-labelled. Cells were lysed and complexes were immunoprecipitated, using anti-γδTCR or isotype control antibodies. Immunoprecipitated proteins were resolved on a reducing 14% SDS-PAGE and transferred to PVDF membrane. Biotinylated proteins were revealed using ABC-HRP and chemiluminescence. Positions of the TCR and CD3 subunits are marked. Material corresponding to the total and to 1/10^th of the material was loaded for eosinophils and γδT cells respectively. Dashed lines indicate that non adjacent lanes of the same gel have been joined on the Figure.