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Analysis of BODIPYFL-Met-tRNA fMet incubated under different conditions by low pH TA SDS-PAGE. BODIPYFL-Met-tRNAfMet samples were hydrolyzed in 0.1 M tris buffers, adjusted to pH values ranging from 6. to 9 by addition of aqueous HCl, at 37 °C for 1.5 hrs. Bands corresponding to BODIPYFL-Met-tRNAfMet and its ester bond hydrolysis products are enclosed in green and orange rectangles, respectively.
